What is the most famous scene in Shakespeare's Twelfth Night?

It's debatable what scene is the most "famous" in Shakespeare's Twelfth Night; various audience members might have different preferences on this matter. Some people are more partial to the more sweepingly romantic scenes within the play, such as in Act One, Scene One, when Count Orsino declares his unrequited love for Lady Olivia and iconically proclaims, "If music be the food of love, play on..." Others prefer the comedic elements, like Sir Toby and Sir Andrew's drunken antics or the ridiculous duel between Sir Andrew and Cesario in Act Three, Scene Four.

That being said, perhaps one of the most loved scenes in the play is Act Three, Scene Four, in which the grumpy servant Malvolio gets his comeuppance at the hands of Maria, Sir Andrew, and Sir Toby. Tired of Malvolio's "better-than-thou" attitude and his eagerness to get the others in trouble, this crew decides to make a fool of Malvolio; they send him a fake love letter from Lady Olivia and convince him that he must appear to her dressed in yellow, cross-gartered stockings.

Malvolio does so and attempts to proclaim his love to a very confused Lady Olivia. This scene is full of miscommunication and hilarious sexual misinterpretations, such as when Olivia asks Malvolio if he'll "go to bed" (here meaning to go lie down and rest in his quarters), and Malvolio perceives it as an invitation to sleep with her, replying, "To bed! ay, sweet-heart, and I'll come to thee."

Malvolio is ultimately humiliated in front of his employer, which is a comedic payoff both for the characters in the play and for the audience who has had to listen to his dry, humorless talk. This scene also includes one of the most quoted Shakespearean lines of all time, which Malvolio repeats from the fake love letter he had received: "Be not afraid of greatness... Some are born great, some achieve greatness, and some have greatness thrust upon them."